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Top 10 Tips for Microbial Limit Test
These tips shall help the analyst to reduce the analytical errors in Microbial Limit Test and help to get accurate and error free results.
The microbial limit test is the quantitative and qualitative assessment of the microbes or microbial contamination test in a sample. This test is performed for:- estimation of the total viable count of fungi and bacteria i.e. Quantitative estimation. Identification of each of the microbes of importance by culturing on selective media i.e. Qualitative estimation.
In quantitative estimation, two tests are carried out, one total fungal count and other is total bacterialcount. For determination of total fungal and total bacterial population size in a given sample. Total four methods are there for microbial limits test: A) Pour Plate Method B) Filtration Method C) Serial Dilution Method D) Spread Plate Method.
Each of these methods has their own error susceptibility but here we will talk in general about avoiding errors. Top 10 tips for the microbial limit test in pharmaceuticals include:
1. Media: For the cultivation of test organism, select the media which favors the fast growth of the microbes. The recommended media are Soybean casein digest broth/agar, and Sabouraud's dextrose agar broth/media.
2. Growth Promotion Capability of the Media: The media we are using for the growth of organisms, we need to choose it wisely. Media need to promote the inoculation of the specific microbes of interest. Antimicrobial effectiveness testing must be done for the product.
3. Time Consumption: Traditional method of Microbial Limit test requires a trained microbiologist analyzing the culture plates after an interval of time. So by using the Growth Direct System, we can reduce the time required for testing by about 50%. This system records the growth of the culture plate every four hours. And it also can detect any contamination. This system analyzes hundreds of culture plates at a time saving the time of microbiologist.
4. Limited Accuracy: Colonies need to grow up to millions of cells before they can be visible to the naked eyes, so a well trained and experienced person may mistake in counting. So the specialist needs to be highly alert and try to avoid errors.
5. Errors of Transfer: Some of the sensitive tests requires serial incubation where samples are delivered from one incubator to another in a specific time at different temperature. So it better to use the Growth Direct system, which automates the process and reduces the error of human effort.
6. Data Entry: After the technician has performed the counting then it is must avoid data entry errors. Technicians can make errors while dealing with hundreds of samples, this error of data entry must be avoided because it will alter the output of the well-performed test.
7. Temperature: Range of temperature must be considered depending on the experimental needs. It depends on the microorganism and the culture media.
8.Atmosphere: Proper atmospheric conditions like aerobic, anaerobic, facultative aerobic/anaerobes, capnophilic and obligate anaerobic conditions must be considered.
9. pH: A appropriate range of pH is required for any microorganism to grow. Any deviation from the pH will inhibit the growth of microbes even if other conditions are favorable. An appropriate pH is required for enzymatic functioning and metabolic activities of microbes.
10. Sterile Conditions: While performing the MLT, it isessential to maintain sterile/aseptic conditions. Any contamination shouldn't invade into the test, otherwise, it would affect the result.
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Teenage life: by a teenager Well we all know that life is very difficult its every stage gives us new lesson but one thing we all can agree on teenage stage is the most difficult one i don't if i can explain it or not but as a teenager i can only try to write my views so here we go
Let's start from the most difficult and complicated one which personally speaking makes you crazy the teenage love life. At this age we all think for once yes he/she is the one. The one with whom we wanted to spent our life but do we really found it . Maybe yes or maybe no i have seen a lot of couples finding true love and they even found it and some breaking up after a relationship of not 1 year but 3-4 years and right now the most trending one falling for your best friend i can't say it's right or wrong but yeah it's fair when both of you were in love but if not than things really become complicated and not to forget the one sided lover phase in which you fall for the guy/girl and at a certain point you tell your feelings but he/she doesn't like you back so you try for some time but eventually at one point you give up and yeah there's one more crush which eventually changes in every week because it is crush and one we all have "the celebrity crush" we all have at least one whether its for sone fictional character or an actor. So that was the one thing but sometimes i just wander do there is something like love do our affections for someone grow that much that we can't think about living without someone or it's just all the fantasies that may exist in books but not in real life but if that so why people say that they love each other just to play with hearts or they really feel it if they feel it so how does they feel for giving your life only on one person does romeo and juliet were true or just someone"s fantasies. Love just made of 4 words but ot complicates life friendship sometimes make someone reckless or the most happiest person on the world and for teenagers i think it's just complicates. So teenage life doesn't end here so like it if you find this useful and wait for next part......
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2015年諾貝爾生理學或醫學獎得主揭曉 寄生蟲疾病新療法研發者摘桂冠 - 風傳媒
2015年「諾貝爾熱季」5日正式展開，首先登場的是諾貝爾生理學或醫學獎（Nobel Prize in Physiology or Medicine，通稱醫學獎...
20155Nobel Prize in Physiology or MedicineWilliam C. Campbell800SEK31201210Stockholm nematodesroundworms400400 85RameltonTrinity College, University of DublinUniversity of WisconsinMadisonDrew University 80IvermectinAvermectins2 842005ArtemisininDihydroartemisinin20119Lasker Award 12
Kun Agüero, la 'ganga' del mercado: tres gigantes compiten por su fichaje - Marca Claro Argentina
La economía de los clubes de fútbol quedó golpeada por el coronavirus y los gastos en fichajes son menores a los de mercados anteriores. Las...
Kun Agüero termina su contrato con Manchester City La economía de los clubes de fútbol quedó golpeada por el coronavirus y los gastos en fichajes son menores a los de mercados anteriores. Las instituciones buscan no fallar en sus contrataciones y también aprovechan los casos en los que jugadores llegan al final de su contrato. Uno de los casos más importantes en Europa es el de Kun Agüero, quien llega al final de su contrato en Manchester City y es seguido por varios equipos. Kun Agüero, de 32 años, queda libre a final de temporada en Manchester City y suena fuertemente en tres equipos: Barcelona, París Saint-Germain y Juventus. El argentino es un futbolista muy experimentado y, que sí está en buenas condiciones físicas, puede ser un gran refuerzo para equipos que buscan pelear cosas importantes. El Barcelona busca la continuidad de Lionel Messi y Kun Agüero se sentaría a escuchar al club catalán si hay una buena propuesta y sí su amigo finalmente se queda en la institución, que ahora tiene como presidente a Joan Laporta. Por su parte, según precisiones de la Gazzetta dello Sport, Juventus está interesado en fichajes ya que esta temporada no se dieron los resultados que deseaban. La directiva busca un nombre que pueda acompañar a Cristiano Ronaldo en ataque y Kun Agüero es una de las opciones más firmes. Los otros candidatos que aparecen en lista son Moise Kean y Arkadiusz Milik. El último interesado de peso es París Saint-Germain. Mauricio Pochettino está interesado en su llegada y el club francés podría ser el que le presente la mejor oferta contractual a Kun Agüero. Según precisiones de Mundo Deportivo, el argentino es uno de los nombres que el entrenador le presentó a la directiva, que estaría muy de acuerdo en intentar su llegada con el pase en su poder, sin necesidad de charlar con Manchester City. Lo cierto es que Sergio Agüero también deberá mejorar en su parte física. La última temporada en Manchester City se vio caracterizada por sus ausencias. En esta campaña con el equipo de Pep Guardiola lleva solo 14 partidos disputados, con tres goles convertidos.
¿Quién tiene más probabilidades de convertirse en Miss Universo 2021? - E! Online
En la cuenta regresiva de la edición 69 del Miss Universo, ya hay candidatas que se acercan a la preciada corona.
Hoy en día, la mayoría de los países han sorteado los límites de la pandemia y han elegido nuevas reinas que los representarán en la reconocida competencia. Luego de meses de incertidumbre, finalmente se conoce cuándo y dónde se llevará a cabo el próximo Miss Universo. La organización reveló que será el próximo 16 de mayo. ¿Dónde? En el Seminole Hard Rock Hotel & Casino de Florida, que destaca por tener forma de guitarra eléctrica.